ABSTRACT
[ ABSTRACT] Rebound depolarization is a special phenomenon of the neurons which generates action potential fol-lowed by a hyperpolarization stimulation.It can be recorded in many kinds of neurons and is the intrinsic membrane charac-teristic of them.Rebound depolarization plays an important role in regulating the firing pattern, rhythmic activity and sy-naptic plasticity of neurons.This review focuses on the basic characteristics, the function and mechanism of the rebound depolarization in physiological and pathological conditions, which provides reference for the clinical treatment of rebound depolarization-related diseases.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of minocycline on hyperpolarization-activated current (Ih) in the substantia gelatinosa (SG) neurons in rat spinal dorsal horn.</p><p><b>METHODS</b>In vitro spinal cord transverse slices were prepared from 3-5-week-old male Sprague-Dawley rats. Using whole-cell patch clamp technique, Ih currents were recorded before and after bath application of minocycline (1-300 µmol/L) to the SG neurons.</p><p><b>RESULTS</b>Ih currents were observed in nearly 50% of the recorded neurons, and were blocked by Ih blocker CsCl and ZD7288. Minocycline rapidly and reversibly reduced the amplitude of Ih and decreased the current density in a concentration-dependent manner with an IC50 of 34 µmol/L.</p><p><b>CONCLUSION</b>Minocycline suppresses the excitability of SG neurons through inhibiting the amplitude and current density of Ih and thereby contributes to pain modulation.</p>